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255: 949Cb9493. Young, J . D.. KO, S . S . , and Cohn, Z. A. (1984) Proc. Natl. Acad. Sci. USA 8 / ; 543Cb5434. , Mori. , and Fukui, Y. (1984) J . Cell Biol. 99: 894899. Zigmond, S . , and Sullivan, S . J . (1979) J . Cell Biol. 82: 517-527. Zigmond, S . , Levitsky, H. I . , and Kreel, B. J . (1981) J . Cell Biol. 82: 517-527. TEKTINS AND MICROTUBULES R. W. Linck OUTLINE I. INTRODUCTION 11. TEKTINS IN CILIARY AND FLAGELLAR MICROTUBULES A. Fractionation of Microtubules into Protofilament Ribbons B.
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Second, Stephens et al. (1989) found that ciliary axonemes from both molluscan gill tissue and sea urchin embryos can be fractionated by a 40°C heat treatment or by 52 R. W. LINCK Figure 9. Localization of tektins in sperm flagellar microtubules by negative stain immuno EM. A and B from S . purpurutus: (A) -sample obtained from low ionic strength dialysis-treated axonemes, incubated with rabbit antibodies to whole tektin filaments, followed by 5 nm gold-conjugated goat anti-rabbit IgG; note, there is little, if any, labeling along the axis of the microtubules or pf- Tektins and Microtubules 53 Figure 10.